Individual and combined cytotoxicity of major trichothecenes type B, deoxynivalenol, nivalenol, and fusarenon-X on Jurkat human T cells - Biosynthèse & Toxicité des Mycotoxines Access content directly
Journal Articles Toxicon Year : 2019

Individual and combined cytotoxicity of major trichothecenes type B, deoxynivalenol, nivalenol, and fusarenon-X on Jurkat human T cells

Abstract

Food and feed commodities are often contaminated by more than one mycotoxin. Among the several combinations that frequently occur, fusariotoxins are often mentioned. The multi-mycotoxins contamination is a serious threat for health. In the present study we investigated the toxic interactions between deoxynivalenol (DON), nivalenol (NIV), and fusarenon-X (FX) in Jurkat T cells by using the MTT assay. The tested mycotoxins alone or in combination had a dose dependent effect on proliferating lymphocytes. According to IC50, it could be classified in decreasing order of toxicity: FX > NIV > DON > DON + FX > NIV + FX > DON + NIV > DON + NIV + FX. The type of mycotoxin interactions was assessed by calculating the combination index (CI) and the dose reduction index (DRI). Our data indicate that an antagonistic effect was strongly observed in binary combination between DON and NIV at higher concentrations and ternary mixtures. Meanwhile, the DON and FX mixtures generated moderate antagonism, while NIV and FX provoked different interactions. The effects of tested mycotoxins on apoptosis were also determined using a FACScan flow cytometer. At IC75, the percentages of apoptotic cells in all treatment groups were significantly different when compared to control group but no significant differences among treatment groups was observed. Taken together, our data suggest that immunotoxicity among multi-mycotoxins contamination cannot be predicted based on individual effects but depends on the mixtures, ratio and/or concentrations in the product, as well as type of target cells.

Domains

Toxicology
Fichier principal
Vignette du fichier
Aupanun et al 2019.pdf (1.11 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-02625366 , version 1 (01-06-2023)

Identifiers

Cite

Sawinee Aupanun, Patchara Phuektes, Saranya Poapolathep, Imourana Alassane-Kpembi, Isabelle P. Oswald, et al.. Individual and combined cytotoxicity of major trichothecenes type B, deoxynivalenol, nivalenol, and fusarenon-X on Jurkat human T cells. Toxicon, 2019, 160, pp.29-37. ⟨10.1016/j.toxicon.2019.02.006⟩. ⟨hal-02625366⟩
35 View
41 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More