Isothermal Nucleic Acid Amplification for Point-of-Care Primary Cervical Cancer Screening

Maryame Lamsisi (1) , Abdelhamid Benlghazi (2) , Jaouad Kouach (2) , Abdelilah Laraqui (3, 2) , Moulay Mustapha Ennaji (4) , Céline Chauleur (5, 6) , Thomas Bourlet (7, 6) , Guorong Li (8, 9, 10)

1 Institut Pasteur du Maroc
2 Hôpital militaire d'instruction Mohammed V [Rabat, Maroc]
3 UM5 - Université Mohammed V de Rabat [Agdal]
4 FSTM - Faculte des sciences et Techniques Mohammedia [Casablanca]
5 SAINBIOSE - Santé Ingénierie Biologie Saint-Etienne
6 CHU ST-E - Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne]
7 GIMAP - Groupe Immunité des Muqueuses et Agents Pathogènes
8 CIRI - Centre International de Recherche en Infectiologie
9 Service des Agents Infectieux et Hygiène [CHU Saint-Etienne]
10 Service d'Urologie - Andrologie [CHU Saint-Etienne]

Human Papillomavirus (HPV) infection is a significant global health concern linked to various cancers, particularly cervical cancer. Timely and accurate detection of HPV is crucial for effective management and prevention strategies. Traditional laboratory-based HPV testing methods often suffer from limitations such as long turnaround times, restricted accessibility, and the need for trained personnel, especially in resource-limited settings. Consequently, there is a growing demand for point-of-care (POC) HPV testing solutions that offer rapid, easy-to-use, and convenient screening at the primary care level. This review provides a comprehensive overview of recent advancements and emerging technologies utilized in POC HPV testing using isothermal amplification methods, in addition to evaluating their diagnostic performance, sensitivity, specificity, and clinical utility compared to conventional laboratory-based assays, particularly in low-resource settings, where access to centralized laboratory facilities is limited. We provide insights into the potential of isothermal nucleic acid amplification to revolutionize cervical cancer screening and prevention efforts worldwide, with emphasis on the need for continued research, innovation, and collaboration to optimize the performance, accessibility, and affordability of POC HPV testing solutions, ultimately contributing to the worldwide effort towards the elimination of this disease.

Mots clés: Lamsisi; M. Benlghazi; A. Kouach; J. Laraqui; A. Ennaji; M.M. Chauleur; C. Bourlet; T. Li; G. Isothermal Nucleic Acid human papillomavirus point-of-care isothermal nucleic acid amplification detection cervical cancer screening; M.; Benlghazi; A.; Kouach; J.; Laraqui; Ennaji; M.M.; Chauleur; C.; Bourlet; T.; Li; G. Isothermal Nucleic Acid human papillomavirus; point-of-care; isothermal nucleic acid amplification; detection; cervical cancer screening

Domaines: Gynécologie et obstétrique

Maryame Lamsisi, Abdelhamid Benlghazi, Jaouad Kouach, Abdelilah Laraqui, Moulay Mustapha Ennaji, et al.. Isothermal Nucleic Acid Amplification for Point-of-Care Primary Cervical Cancer Screening. Viruses, 2024, 16, ⟨10.3390/v16121852⟩. ⟨hal-04822104⟩