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Protein interaction with SiO2 and AgNPs: from adsorption on solid surfaces to organization and conformational changes

Marvine Soumbo 1, 2 Christina Villeneuve-Faure 3 Caroline Bonafos 4 Christine Roques 2 Kremena Makasheva 1 
1 LAPLACE-ScIPRA - Sciences et Ingénierie des Plasmas Réactifs et des Arcs
LAPLACE - LAboratoire PLasma et Conversion d'Energie
3 LAPLACE-DSF - Diélectriques Solides et Fiabilité
LAPLACE - LAboratoire PLasma et Conversion d'Energie
4 CEMES-NeO - Nano-Optique et Nanomatériaux pour l'optique
CEMES - Centre d'élaboration de matériaux et d'études structurales
Abstract : Driven by many applications, the development of new biomaterials has considerably increased in the last decade. The current research strategies also involve revealing of the relationship between protein structure and function due to the exposure and interaction of proteins with non-biological organic and inorganic solid surfaces. Aiming at understanding of the mechanisms of protein adsorption on solid surfaces we follow in this work the organization dynamics of proteins (Bovine Serum Albumin, BSA and Fibronectin, Fn) adsorbed on thin silica layers without or with silver nanoparticles (AgNPs), deposited on their surfaces. It is found that although starting with the same protein concentration in solution (0.05 g/L), the adsorbed amount of proteins on SiO2 surfaces is twice larger for Fn (1.32 µg/cm 2) compared to BSA (0.58 µg/cm 2). The proteins adopt different conformations according to the surface pattern. On a flat SiO2 surface, the BSA proteins organize in a lace-like network while the Fn proteins adopt a branching-type. The patterned by AgNPs surfaces induce conformational changes of the proteins. In interaction with AgNPs both types of proteins fold up to attain mainly compact globular conformation.
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https://hal.archives-ouvertes.fr/hal-03578945
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Submitted on : Thursday, February 17, 2022 - 4:33:39 PM
Last modification on : Monday, July 4, 2022 - 9:58:43 AM
Long-term archiving on: : Wednesday, May 18, 2022 - 7:17:41 PM

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Marvine Soumbo, Christina Villeneuve-Faure, Caroline Bonafos, Christine Roques, Kremena Makasheva. Protein interaction with SiO2 and AgNPs: from adsorption on solid surfaces to organization and conformational changes. 2021 IEEE 16th Nanotechnology Materials and Devices Conference (NMDC), Dec 2021, Vancouver, Canada. pp.1-4, ⟨10.1109/NMDC50713.2021.9677562⟩. ⟨hal-03578945⟩

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